Pharmacological and surgical experimental animal models of induction of spermatogenesis disorders

Introduction. Experimental induction of spermatogenesis disorders is possible mainly by physical, pharmacological methods. However, not all methods can cause non-obstructive azoospermia. Objective. To evaluate and compare the effectiveness in rat models applying ligatures to spermatic cords administration cisplatin. Materials & . Seventy-three mature male rats (Wistar) were divided into 2 experimental groups 1 control (n = 9) group: group 27) with ligature on cord for 12 h 9), 24 36 9); 37) five-fold intraperitoneal cisplatin at concentrations 5 mg/kg, 3 mg/kg. On days 0, 7, 14, 28 after last day disorders, epididymal semen analysis, blood test, total serum testosterone, pathomorphological examination testes tissue, body weight, reproductive system organ weight performed assess model performance. Results. Ligation did have a negative effect general condition animals (p < 0.05), test 0.05); there was decrease testicular appendage testis prostate seminal vesicles change > 0.05). In 1, number epidermal spermatozoa decreased subgroups, statistically significant changes recorded 7 (exposure h) 12, research. Histologically, no inhibition spermatogenesis, except area, diameter tubules surgery 24, h). 2, survival noted only when using dose mg/kg five times. Body without recovery, thrombocytopenia wk, leukocytopenia regressed wk study. A organs noted. Sperm concentration recovered wk. analysis biopsies: pronounced disorganization spermatogenic epithelium, absolute area tubules. Conclusion. does permanent spermatogenesis. Cisplatin causes persistent severe damage epithelium.